Science and Technology of Energetic Materials

Vol.65, No.3 (2004)


Study on biodegradation of 2, 4, 6-trinitrotoluene by Pseudomonas sp. strain TM15 (*in japanese)
Toshinari Maeda, Kiwao Kadokami, and Hiroaki I. Ogawa


We isolated and identified a pseudomonad strain named as Pseudomonas sp. strain TM15 from soils in Yamada Green Zone, Kitakyushu City where 2, 4, 6-trinitrotoluene (TNT) specifically has been detected. This strain can efficiently biotransform TNT in comparison with other isolating bacteria. TNT was converted to two kinds of mono-amino-dinitrotoluene, 2-amino-4, 6-dinitrotoluene and 4-amino-2, 6-dinitrotoluene, further to benzoate and catechol. Based on the extinction of the absorbance derived from aromatic ring (260-280 nm), this bacterium was finally expected to cleavage the ring of TNT. The results of umu test using the bioluminescent bacterium showed that the genotoxicity from TNT was eliminated by Pseudomonas sp. strain TM15.

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